Cinnamomum burmannii EXTRACT AMELIORATES HIGH GLUCOSE-INDUCED BRAIN APOPTOSIS IN ZEBRAFISH EMBRYOS THROUGH INHIBITION OF PROCASPASE-9 : IN SILICO AND IN VIVO STUDY

Background: Brain is an organ that prone to oxidative stress and subsequent apoptosis due high aerobic metabolism relatively low antioxidants, especially under hyperglycemic condition. Cinnamomum burmanii (CB) a species abundant in Indonesia, therefore it of special concern for researchers identify the anti-apoptotic effect CB. Objective: This study was initiated determine CB extract on inhibition brain zebrafish embryos exposed glucose investigate its anti-apoptosis mechanism by molecular docking approach. Methods: Molecular conducted interaction between several extracts main constituents with target protein procaspase-9, compared control ligand Saxagliptin. Zebrafish were used assess 4% exposure three doses treatment (1.25, 5, 10 µg/ml) region. High-glucose condition embryo confirmed overexpression Phosphoenolpyruvate carboxykinase (PEPCK). Apoptosis evaluated performing acridine orange (AO) staining quantified ImageJ software. Results: indicated compounds, namely epicatechin, displayed stronger interactions procaspase-9 There increased numbers apoptotic cells seen around region glucose-treated group. Meanwhile, supplementation at dose µg/ml resulted decreased amount Conclusion: The results suggest protects from hyperglycemic-induced modulating procaspase-9.